Review





Similar Products

tetramethylrhodamine tmr halotag ligand  (MedChemExpress)
94
MedChemExpress tetramethylrhodamine tmr halotag ligand
Tetramethylrhodamine Tmr Halotag Ligand, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tetramethylrhodamine tmr halotag ligand/product/MedChemExpress
Average 94 stars, based on 1 article reviews
tetramethylrhodamine tmr halotag ligand - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
halotag ligands sarafluor 650t sf650t  (GORYO Chemical)
95
GORYO Chemical halotag ligands sarafluor 650t sf650t
Halotag Ligands Sarafluor 650t Sf650t, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/halotag ligands sarafluor 650t sf650t/product/GORYO Chemical
Average 95 stars, based on 1 article reviews
halotag ligands sarafluor 650t sf650t - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
halotag ligands sarafluor 650 t sf650t  (GORYO Chemical)
95
GORYO Chemical halotag ligands sarafluor 650 t sf650t
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Halotag Ligands Sarafluor 650 T Sf650t, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/halotag ligands sarafluor 650 t sf650t/product/GORYO Chemical
Average 95 stars, based on 1 article reviews
halotag ligands sarafluor 650 t sf650t - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
sarafluor 650b sf650b ligands  (GORYO Chemical)
95
GORYO Chemical sarafluor 650b sf650b ligands
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Sarafluor 650b Sf650b Ligands, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sarafluor 650b sf650b ligands/product/GORYO Chemical
Average 95 stars, based on 1 article reviews
sarafluor 650b sf650b ligands - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
biotin labeled halotag fusion proteins  (IBA Lifesciences)
96
IBA Lifesciences biotin labeled halotag fusion proteins
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Biotin Labeled Halotag Fusion Proteins, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin labeled halotag fusion proteins/product/IBA Lifesciences
Average 96 stars, based on 1 article reviews
biotin labeled halotag fusion proteins - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
halotag sarafluor 650t ligand  (GORYO Chemical)
95
GORYO Chemical halotag sarafluor 650t ligand
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Halotag Sarafluor 650t Ligand, supplied by GORYO Chemical, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/halotag sarafluor 650t ligand/product/GORYO Chemical
Average 95 stars, based on 1 article reviews
halotag sarafluor 650t ligand - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
a201-01  (goryo chemical)
94
goryo chemical a201-01
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
A201 01, supplied by goryo chemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a201-01/product/goryo chemical
Average 94 stars, based on 1 article reviews
a201-01 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
crl 3216 t7 express e coli neb c2566h recombinant dna halotag app mneongreen gershlick lab  (New England Biolabs)
96
New England Biolabs crl 3216 t7 express e coli neb c2566h recombinant dna halotag app mneongreen gershlick lab
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Crl 3216 T7 Express E Coli Neb C2566h Recombinant Dna Halotag App Mneongreen Gershlick Lab, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crl 3216 t7 express e coli neb c2566h recombinant dna halotag app mneongreen gershlick lab/product/New England Biolabs
Average 96 stars, based on 1 article reviews
crl 3216 t7 express e coli neb c2566h recombinant dna halotag app mneongreen gershlick lab - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
magnetm halotag® beads  (Promega)
90
Promega magnetm halotag® beads
A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having <t>SF650T-Halo-MIM</t> I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.
Magnetm Halotag® Beads, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/magnetm halotag® beads/product/Promega
Average 90 stars, based on 1 article reviews
magnetm halotag® beads - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having SF650T-Halo-MIM I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.

Journal: Nature Communications

Article Title: Efficient cellular transformation via protein delivery through the protrusion-derived extracellular vesicles

doi: 10.1038/s41467-025-66351-1

Figure Lengend Snippet: A Schematic illustration of EV endocytosis, endosomal transport, and cargo release. B 3D-PALM/STORM live cell imaging of the CIP4-mEos4b (magenta)-expressing CIP4 KO PANC-1 cells with l-EV fraction having SF650T-Halo-MIM I-BAR (green). Images were recorded every 10 s. Scale bar, 500 nm. C Colocalization of CIP4-mEos4b with SF650T-Halo-MIM I-BAR in ( B ). The percentages of voxels co-localized in each frame were calculated (“RAW”). Co-localization of the CIP4 images with the 180°-rotated SF650T-Halo-MIM images is shown as “Randomized”. The 13 super-resolution images from independently seeded 5 cells, which resulted in 4, 1, 4, 2, and 2 images, respectively, were analyzed. D Colocalization of EVs from the l-EV fractions having both Halo-Rac1 and mCherry-MIM I-BAR with EEA1 (Top) or Lamp1 (Bottom) in WT cells after 3 h of incubation, in which 10 6 cells were treated with 1.6 × 10 9 EV/ml in 1 ml. Arrowheads in the enlarged images show their colocalization. Scale bars, 10 μm. E Quantification of the colocalization of Halo-Rac1 with EEA1 (Top) or Lamp1 (Bottom) in WT and CIP4 KO cells using Mander’s coefficients after EV addition. The EV fraction and the number of cells and EVs were the same as ( D ). The red lines indicate the means of 54 cells from 3 independent experiments. F PALM imaging of the l-EVs having SF650B-Halo-Rac1 in the late endosome, having Lamp1-mEos4b (Left). Enlargements of the square regions in the left images are on the Right. Arrowheads indicate possible release of SF650B-Halo-Rac1 from the endosome. Scale bars, 10 μm (Left) and 100 nm (Right). G Quantification of the Halo-Rac1 release from the endosome in ( F ). The percentage of EVs with crossover of Halo-Rac1 and Lamp1 in a cell is shown (17 cells from 3 independent experiments). All EVs with Lamp1-positive endosomes were counted. The full range of images with the marking of the counting of the EVs is shown in Supplementary Fig. . The percentage of Halo-Rac1-releasing EVs was calculated as the percentage of “Halo-Rac1-releasing EVs” among the Halo-Rac1 EVs in the Lamp1-positive endosomes (endosome-colocalizing EVs). The means ± SD are shown. Statistical significance was determined by a two-tailed unpaired Mann–Whitney U test ( C , E ) or a two-tailed t-test with Welch’s correction ( G ). Source data are provided as a file.

Article Snippet: To label Halo7-tagged proteins in the EVs, HaloTag ligands SaraFluor 650 T (SF650T) or SaraFluor 650B (SF650B) ligands (Goryochemical, #A308-01 or #A201-01) were used.

Techniques: Live Cell Imaging, Expressing, Incubation, Imaging, Two Tailed Test, MANN-WHITNEY